A major limitation of MRM is the number of targets that can be measured in one run. If the peptide retention times are known this number can be increased by scheduling the acquisition. 

The use of iRT in scheduled LC-MRM experiments results in higher accuracy of retention time prediction compared to in-silico methods.

Calibrating MRM assays with our iRT-Kit™ will dramatically increase the throughput of your MRM experiments, even up to 10 times, by bundling more transitions into one run.

The kit integrates our iRT calculator, that makes iRT easy to use across various LC systems and gradients. iRT values can easily be stored in databases and shared across laboratories.

Downloads: iRT-Kit reference sheet for transitions and sequence information.
Manuals: Quick Reference and the Safety Data Sheet.